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Description
Time-lapse microscopy of histone-RFP expressing mouse oocytes injected with siTPX2-5h, a doublestranded siRNA against TPX2, (targeting protein for Xenopus kinesin-like protein, xklp2). Images were taken every 20 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner. Video S6 Found at: doi:10.1371/journal.pone.0003338.s006
Technical Details
Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
Biological Sources
- NCBI Organism Classification
- Mus musculus
- (house mouse)
- Cell Type
- oocyte
- Cellular Component
- microtubule
- microtubule organizing center part
- nuclear chromosome
Biological Context
- Biological Process
- meiotic spindle organization
- microtubule organizing center organization
- RNA interference
- Molecular Function
- GTPase activity
Attribution
- Names
- Stephane Brunet
- Julien Dumont
- Karen W. Lee
- Kazuhisha Kinoshita
- Pascale Hikal
- Oliver J. Gruss
- Bernard Maro
- Marie-Helene Verlhac
- Link
- http://www.plosone.org/article/info:do...
- Pubmed
- 18833336
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- confocal microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- Visualization Methods
- DsRed
- Processing History
- unprocessed raw data
- Data Qualifiers
- raw, unprocessed data
Sample Preparation
- Methods
- living tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 150px | —— |
| Y | 150px | —— |
| Time | 900 sec | 53 |
*CIL – Cell Image Library accession number. Please use this to reference an image.