Time-lapse microscopy of histone-RFP expressing mouse oocytes injected with siTPX2-5h, a doublestranded siRNA against TPX2, (targeting protein for Xenopus kinesin-like protein, xklp2). Images were taken every 20 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner. Video S6 Found at: doi:10.1371/journal.pone.0003338.s006
Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
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