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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35377*  Cite 

Whole human female metaphase chromosome from metaphase arrested cell swollen in hypotonic medium, and treated to remove histones. The residual 'scaffold' was imaged with high voltage EM at 1 MeV. This image was taken with a specimen tilt of 54 degrees. Grouped with it is an image of the same area at 46 degrees tilt, providing an oblique stereo view of the chromosome.

Technical Details

HeLa cells were metaphase-arrested by a 3hr treatment with colcemid, the cells mechanically broken by passage through a 22-gauge needle and exposed to hypotonic 75 mM KCl, treated to remove histones, spread on a water surface, fixed with formaldehyde, picked up on formvar coated grid, critical point dried, and imaged with the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic microscopy of chromosomes. Meth Cell Biol 22:77-96.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
cevical carcinoma
Cell Line
Cellular Component
condensed chromosome
Biological Context
Biological Process
nuclear division
Hans Ris
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
visualization of contiguous regions
uranyl salt
Processing History
unprocessed raw data
Sample Preparation
formaldehyde fixed tissue
critical_point dried specimen
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 5770px 1.5µm
Y 2333px 1.5µm