Description

Living Lilium pollen tube labeled with carboxy-dichlorofluorofluorescein (green) to mark the vacuole and cytochalasin D TMR to mark endoplasmic reticulum (red) imaged using laser scanning focal microscopy. Shown is a central 1.0 micron thick x-y slice. The growing tip is devoid of both organelles. Other images in the group reveal the distributions of additional cytoplasmic components.

Technical Details

Living lily pollen tubes were labeled and then imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Green and red signals from a central plane through the tube were merged to generate the image. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.