Living Lilium pollen tube labeled with TMRE (red)to mark mitochondria and imaged using laser scanning focal microscopy. Shown is a central 1.0 micron thick x-y slice. The growing tip is devoid of mitochondria. Other images in the group reveal the distributions of additional cytoplasmic components.
Living lily pollen tubes were labeled and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. The red signals from a central plane through the tube is shown. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.