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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35293*  Cite 
Description

Living Lilium pollen tube labeled with paclitaxel (blue) to mark microtubules (blue) and cytochalasin D TMR to mark endoplasmic reticulum (green) imaged using laser scanning focal microscopy. The growing tip is devoid of both organelles. Other images in the group reveal the distributions of additional cytoplasmic components.

Technical Details

Living lily pollen tubes were labeled and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Green (endoplasmic reticulum) and blue (microtubules) signals from a central plane through the tube were merged to generate the image. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.

Biological Sources
NCBI Organism Classification
Lilium formosanum
Cell Type
pollen tube
Cellular Component
microtubule
endoplasmic reticulum
Biological Context
Biological Process
pollen tube growth
Attribution
Names
Alenka Lovy-Wheeler
Luis Cardenas
Joseph Kunkel
Peter K Hepler
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35293
Archival Resource Key (ARK)
ark:/b7295/w9cil35293
Grouping This image is part of a group.
Imaging
Image Type
photomultiplier tube (PMT)
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
Visualization Methods
oregon green paclitaxel
Tetramethyl rhodamine (TRITC)
Processing History
merged confocal data
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 389px ——
Y 174px ——