The Cell: An Image Library - Image CIL:35290

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CIL:35290^*

Description

Freeze substituted Lilium pollen tube immunolabeled for microtubules (blue>magenta) and imaged using laser scanning confocal microscopy. Shown is a projection of x-y slices revealing the distribution of microtubules in the sub-apical region. This group of images also contains an x-z projection of the same specimen.

Technical Details

Germinated lily pollen on agar loops was plunge frozen, freeze substituted, labeled with an anti-tubulin mouse monoclonal antibody followed by Cy-3 goat anti-mouse and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. The image shown is a projection of x-y slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.

Biological Sources

NCBI Organism Classification: Lilium formosanum; (St Joseph's lily)
Cell Type: pollen tube
Cellular Component: microtubule

Biological Context

Biological Process: pollen tube growth

Attribution

Names: Alenka Lovy-Wheeler; Kathleen L Wilsen; Tobias I Baskin; Peter K Hepler

Grouping

This image is part of a group.

Imaging

Image Type: photomultiplier tube (PMT)
Imaging Mode: confocal microscopy
Parameters Imaged: distribution of epitope
Source of Contrast: distribution of a specific protein
Visualization Methods: Cy3
Processing History: projection of confocal slices

Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: cryofixed tissue; FBbi:00000621
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2001px	——
Y	424px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.