Living Lilium pollen tube labeled with dichloro-flourescein diacetate to mark the vacuole and imaged using laser scanning focal microscopy. Shown is a central 1.0 micron thick x-y slice.The growing tip is devoid of vacuole. Other images in the group reveal the distributions of additional cytoplasmic components.
Living lily pollen tubes were labeled and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Image shows a central x-y plane through the tube. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.