Fixed Lilium pollen tubes were treated with fluorescent phalloidin to label filamentous actin, and confocal images obtained. A strongly labeled actin fringe near the growing tip is present. Other images in the group reveal the distributions of additional cytoskeletal elements and membranous components.
Germinated lily pollen was fixed with paraformaldehyde and glutaraldehye, treated with Alexa-543 phalloidin to label actin, and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Image is a projection of several confocal x-y slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.