Freeze substituted Lilium pollen tube immunolabeled for filamentous actin and imaged using laser scanning confocal microscopy. Shown is a projection of x-z slices revealing the cortical distribution of the dense sub-apical actin fringe. This image group also contains a projection of x-y slices of this specimen.
Germinated lily pollen on agar loops was plunge frozen, freeze substituted, labeled with an anti-actin mouse monoclonal antibody followed by Cy-3 goat anti-mouse and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. The image shown is a projection of x-z slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.
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