The Cell: An Image Library - Image CIL:35285

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CIL:35285^*

Description

Freeze substituted Lilium pollen tube immunolabeled for filamentous actin and imaged using laser scanning confocal microscopy. Shown is a projection of z-slices revealing the dense sub-apical actin fringe. Other images in the group reveal the distributions of other cytoskeletal elements and membranous components.

Technical Details

Germinated lily pollen on agar loops was plunge frozen, freeze substituted, labeled with an anti-actin mouse monoclonal antibody followed by Cy-3 goat anti-mouse and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. The image shown is a projection of z-slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.

Biological Sources

NCBI Organism Classification: Lilium formosanum; (St Joseph's lily)
Cell Type: pollen tube
Cellular Component: actin cytoskeleton

Biological Context

Biological Process: pollen tube growth

Attribution

Names: Alenka Lovy-Wheeler; Kathleen L Wilsen; Tobias I Baskin; Peter K Hepler

Grouping

This image is part of a group.

Imaging

Image Type: photomultiplier tube (PMT)
Imaging Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: Cy3
Processing History: projection of confocal slices

Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: cryofixed tissue; FBbi:00000621
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	532px	——
Y	216px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.