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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35201*  Cite 

Movie showing motility and morphology of cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) arrested in G1 using 0.08 uM nocodazole (Uetake and Sluder 2010, Curr Biol 20:1666-1671). The time series used to prepare the movie is grouped in the Cell Image Library.

Technical Details

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 10x NA 0.3 phase contrast objective lens. Images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using HC Image software.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
retinal pigment epithelium
Cell Line
Biological Context
Biological Process
cell motility
cell morphology
Yumi Uetake
Greenfield Sluder
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 1344px 0.645µm
Y 1024px 0.645µm
Time 180 seconds 1868