Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35166^* Cite

Description

Constitutive expression of GFP (Green Fluorescent protein) in MTLn3 cells, a metastatic rat breast carcinoma line. This time lapse sequence of a single optical section near the substrate shows the cells jostling and the intrinsic movement of organelles that appear dark because they do not contain the fluorescent protein. This cell line is useful both as a cell culture system and also as a source of fluorescent cells that may be used to study metastasis in live rats or mice.

Technical Details

BioRad Radiance 2000 laser scanning confocal microscope with Kr/Ar laser for excitation of permanently transfected GFP at 488 nm. The pixel size is approximate.

Biological Sources

NCBI Organism Classification: Rattus rattus
Cell Type: permanent cell line cell
Cell Line: MTLn3
Cellular Component: cytosol

Biological Context

Biological Process: interphase

Attribution

Names: Maryse Bailly; Michael Cammer

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35166
Archival Resource Key (ARK): ark:/b7295/w9cil35166

Imaging

Image Type: single-spot confocal microscopy
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.16µm
Y	512px	0.16µm

Time	20 seconds	40