Live mitotic HeLa cell treated with epsin1 siRNA, DiOC6(3)to label mitotic membranes (green), and Hoechst 33258 to label chromosomes (blue). Confocal images were taken at 0.118 μm steps along the Z-axis. Epsin1 depleted cells show aberrant membrane morphologies compared with control cells (see grouped image).
A Leica TCS SP5 DM6000B confocal laser scanning microscope with HCX Plan Apo 63x/1.4 oil objective lens was used to capture the images.
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