The Cell: An Image Library - Image CIL:35127

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CIL:35127^*

Description

Primary bone marrow macrophages derived from mouse plated on a coverslip and imaged live. Phase contrast microscopy allows us to see the dense f-actin rich leading edge, ruffles, mitochondria, the nucleus, and other organelles throughout the volume of the cells. At the center of the image is a polarized lamellipod of a crawling cell. The glass micropipette at the bottom of the image is delivering CSF (colony stimulating factor) locally and the cell may be responding.

Technical Details

Live bone marrow macrophages plated in MatTek dish and imaged with a 60X N.A. 1.4 phase 3 Olympus objective on an IX71 microscope with a Cooke Sensicam QE camera and IPLab software running on a Dell Windows XP computer.

Biological Sources

NCBI Organism Classification: Mus musculus; (house mouse)
Cell Type: macrophage
Cell Line: Bone marrow
Cellular Component: ruffle membrane

Biological Context

Biological Process: ruffle organization
Molecular Function: chemokine receptor activity
Mouse Gross Anatomy: TS24,femur

Attribution

Names: Michael Cammer; Dianne Cox

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: phase contrast microscopy
Parameters Imaged: elastic scattering of photons
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1376px	0.11μm
Y	1040px	0.11μm

*CIL – Cell Image Library accession number. Please use this to reference an image.