The Cell: An Image Library - Image CIL:35065

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CIL:35065^*

Description

Electron micrograph of keratocyte or fibroblast lamellipodial actin network after unprotected extraction. All examples demonstrate frequent branching of actin filaments. Image corresponds to a single panel from Figure 2d in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 2d can be found as CIL 35060-35068.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

Cellular Component: actin cytoskeleton

Biological Context

Biological Process: branching of actin filaments; actin filament-based process
Molecular Function: structural constituent of cytoskeleton

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Visualization Methods: shadowing and plating; platinum replica
Processing History: unprocessed raw data

Sample Preparation

Methods: permeabilized tissue; glutaraldehyde fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	453px	——
Y	457px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.