Alternate header for print version


Licensing
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34923*  Cite 
Description

Localization of cross-linking proteins in fibroblast cytoskeleton. Fluorescence microscopy and corresponding intensity profiles human 356 fibroblast lamellipodia double stained with TRITC-phalloidin (red) and ABP-280 (green). The protein/actin ratio at the leading edge of the lamellipodium is medium for ABP-280. Immuno-EM of the cell edge (CIL 34926) or interior (CIL 34929) of cytochalasin D treated human 356 fibroblasts stained with ABP-280 primary antibody and 10-nm gold-conjugated secondary antibody reveals ABP-280 at filament crossovers in the cell interior. Image corresponds to Figure 5b and b' from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast
Cell Line
human 356
Cellular Component
lamellipodium
actin cytoskeleton
ABP-280
Biological Context
Biological Process
cellular localization
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL34923
Archival Resource Key (ARK)
ark:/b7295/w9cil34923
Grouping This image is part of a group.
Sample Preparation
Methods
glutaraldehyde fixed tissue
permeabilized tissue
phaloidin
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 783px 0.132µm
Y 678px 0.132µm