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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34922*  Cite 
Description

Localization of cross-linking proteins in fibroblast cytoskeleton. Fluorescence microscopy and corresponding intensity profiles of Xenopusfibroblast lamellipodia double stained with TRITC-phalloidin (red) and either p21 (Arp 2/3) antibodies (green). The protein/actin ratio at the leading edge of the lamellipodium is high for Arp2/3 complex. Immuno-EM of the cell edge (CIL 34925) or interior (CIL 34928) of cytochalasin D-treated Xenopus fibroblasts stained with p21 primary antibody and 10-nm gold-conjugated secondary antibody reveal Arp2/3 complex at Y-junctions. Image corresponds to Figure 5a and a' from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
fibroblast
Cellular Component
lamellipodium
actin cytoskeleton
Arp2/3 protein complex
Biological Context
Biological Process
cellular localization
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL34922
Archival Resource Key (ARK)
ark:/b7295/w9cil34922
Grouping This image is part of a group.
Sample Preparation
Methods
glutaraldehyde fixed tissue
permeabilized tissue
polyethylene glycol
methanol fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 775px 0.105µm
Y 684px 0.105µm