Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34848^* Cite

Description

Time lapse movie of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using DIC optics. This movie and the stack of images used to prepare it are grouped in the Cell Image Library.

Technical Details

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 100x HCX PLAN APO NA 1.35 objective lens. images were captured at 30 sec intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: retinal pigment epithelium
Cell Line: hTERT-RPE1

Biological Context

Biological Process: mitosis; cell proliferation

Attribution

Names: Toshiyuki Hatano; Greenfield Sluder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34848
Archival Resource Key (ARK): ark:/b7295/w9cil34848

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1344px	0.0426µm
Y	1024px	0.0246µm

Time	30 seconds	1079