A micrograph showing the polyploidal macronucleus of Tetrahymena containing many copies of the genome. Chromatin material is found as condensed electron opaque spots as well as in an uncondensed form between the opaque spots. The macronucleus, unlike the micronucleus, contains many nucleoli that tend to accumulate next to the nuclear envelope. Nucleoli are sites of ribosomal RNA synthesis and ribosome subunit assembly. TEM taken on 8/14/67 by R. Allen with Philips 200 operating at 60kV. Neg. 4,500X. Bar = 1µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:39208) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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