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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34604^* Cite

Description

This cross section is near the proximal end of the cilium. Triangular densities link the doublets to the membrane. TEM taken 7/23/65 by R. Allen with RCA EMU3F operating at 50kV. Adapted with permission. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Tetrahymena pyriformis
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist

Attribution

Names: Richard Allen (University of Hawaii)
Published: J. Cell Biol. 40:716-733, 1969
Pubmed: 5765762

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34604
Archival Resource Key (ARK): ark:/b7295/w9cil34604

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	968px	——
Y	1329px	——