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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34541*  Cite 

Exposure of a cortical neuron to netrin induces the phosphorylation of focal adhesion kinase (FAK) at Tyr397 along the axon and within the growth cone (gc). Insert shows the control neuron (without treatment). A day-in-vitro 3 (DIV3) cultured cortical neuron was exposed to 100 ng/ml netrin for 30 minutes, fixed with 4% paraformaldehyde (PFA), then immunostained with anti-phospho-FAK (Tyr397) antibody (green) and Texas Red-X phalloidin (red). Scale bars, 20 µm; insert, 50 µm.

Technical Details

Cortical neurons were isolated and cultured from E17 embryo using a standard protocol as described in Lehnardt et al.,Proc Natl Acad Sci U S A, 100 (14): 8514-9. At DIV3, neurons were treated with 100 ng/ml netrin for 30 minutes, fixed with 4% PFA, then stained with anti-phospho-FAK (Tyr397) (Upstate) antibody and Texas Red-X phalloidin (red). Images were digitally acquired using a fluorescence microscope (Nikon Eclipse 660) equipped with Spot cooled CCD camera (Diagnostic Instruments).

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
CNS neuron (sensu Vertebrata)
Cellular Component
growth cone membrane
Biological Context
Biological Process
neuron development
Molecular Function
netrin receptor activity
Yinghua Ma
Timothy Vartanian
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
distribution of a specific protein
Visualization Methods
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 3396px 0.027µm
Y 4000px 0.027µm