The Cell: An Image Library - Image CIL:34532

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

Comments:

Add to Photobox

CIL:34532^*

Description

High resolution image of the cytoplasm of Tetrahymena. Mitochondria have tubular cristae, infoldings of their inner membranes. Peroxisomes, with a few tubular inclusions, mucocysts, and a planar view of rough ER, to which many polysomal ribosome arrays are attached, fill the cytosol. TEM taken on 2/11/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).