The video is a representative movie of baceria incubated in DMEM for 3 h on top of an array of pilars of stiffness 500 pN/micron. The video is real time and shows the behavior of one or two bacteria for 23 s after 3 h of incubation. The video relates to CIL: 34522 and 34523, which show bacteria incubated in different media. The pilars are 3 micron apart center to center. The video is DIC microscopy with the focus on the top of the pilars. See the already deflected pillars around the bacterium and both transient and sustained pulls. This video corresponds to Video 2 from PLoS Biol. 2008 Apr 15;6(4):e87.
A pulling force exerted on a pillar, such as that imposed by an attached pilus fiber, causes a displacement of the pillar tip. The displacement can be correlated to the force applied on the pillar after proper calibrations. All optical microscope images were obtained on conventional inverted microscopes (either Olympus IX 71 or Zeiss Axiovert 100).
|Spatial Axis||Image Size||Pixel Size|