Alternate header for print version

Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:33796*  Cite 

Tissue section of human prostate containing adenocarcinoma that has been immunostained for the cell-surface antigen CD74. Nuclei are stained in blue. This image is part of a large collection of images generated from numerous specimens to characterize the distribution of CD74 in human prostate tissue. A summary of the entire data set is provided below.

Cell Type % intense % equivocal % none # assays
Atrophic glands 0 0 100 1
Hyperplastic glands 0 0 100 1
Normal glands 0 0 100 1
Basal Epithelial Cells 35 30 35 1
Stromal Endothelial Cells 75 10 15 1
Stromal Fibromuscular Cells 0 0 100 1
Stromal Nerve Sheath Cells 0 0 100 1
Stromal Perineural Cells 0 0 100 1

The table indicates the percent of each cell type with the indicated staining intensity. Staining intensity levels were classified as intense, equivocal (weak or very focal) or none. Not all of the cell types and staining patterns are visible in each image.

This image is part of a large collection of immunohistochemistry images of cell-surface antigens generated by the SCGAP Urologic Epithelial Stem Cells (UESC) Project. The overall goal of the project is to characterize and isolate epithelial stem cell populations from two urologic organs, the prostate and bladder. Links are provided below for the UESC Project database, the entire human prostate immunostain summary, the CD74 immunostain summary, and information on the specimen that this image is from. Other images of CD74 human prostate immunostains are accessible following the group link.

Technical Details

The "Image ID" for this image is provided in the Attribution section. Because the images are systematically named, information such as the antibody used in staining, the organism, tissue type, tissue block, and magnification are all encoded in the Image ID. As an example, the image 'CD44 98-395F HP ba 100.jpg' was stained with anti-CD44, was derived from human prostate tissue block 98-395F, is human prostate tissue (HP), and an image of microscopic field of view b within field a was captured at 100× magnification.

Complete technical details available in the Protocol Section of SCGAP UESC website (link provided). Briefly, radical prostatectomy specimens were transverse-sectioned 3mm thick and tissue blocks were embedded in precooled OCT (Tissue-tek) medium. Frozen tissue blocks were thin-sectioned (5µ) and fixed in cold acetone. A monoclonal primary antibody (PharMingen) was used followed by a biotinylated secondary antibody. Bound antibody was detected using avidin-biotin-peroxidase (Vector Labs) and diaminobenzidine (DAB). Sections were lightly counterstained with hematoxylin. Images were acquired using a digital camera and either an Olympus BX41 or Olympus BH2 microscope.

Biological Context
Human Disease
prostate adenocarcinoma
Larry True
Eric Deutsch
Laura Pascal
Tracy Sherertz
Laura Walashek
David Campbell
Alvin Liu
SCGAP UESC (Urologic Epithelial Stem Cells) P...
UESC Database Publication
Image ID: CD74 99-060D HP ba 100
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
widefield illumination
bright-field microscopy
Parameters Imaged
absorption of illumination
Source of Contrast
differences in adsorption or binding of stain
distribution of a specific protein
differences in intrinsic optical density
Visualization Methods
primary antibody plus labeled secondary antibody
3,3'-diaminobenzidine (DAB)
horseradish peroxidase
biotin-avidin amplification
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Spatial Axis Image Size Pixel Size
X 1600px ——
Y 1200px ——