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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:32023*  Cite 
Description

Neural plate of Xenopus laevis at early neural tube closure.

Technical Details

Fluorescent fusion proteins encoded by mRNAs, transcribed in vitro, are used to label appropriate portions of cells (i.e., chromosomes via H2B-GFP or membranes). Mixes of mRNA are microinjected with glass needles directly into the embryo at the four-cell stage by use of a picospritzer (see 1 in the figure). For analysis of the early neural plate, the two dorsal cells are injected. Embryos are then grown to stage 13 for early neural plate development. Embryos were imaged on a Zeiss LSM 510 using a 40X objective.

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
neurecto-epithelial cell
Cellular Component
nucleus
Biological Context
Biological Process
cytokinesis
neural tube closure
Attribution
Names
Esther K. Kieserman
Michael Glotzer
John B. Wallingford
Published
Current Biology, 18, 116-123. 2008
Pubmed
18207743
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL32023
Archival Resource Key (ARK)
ark:/b7295/w9cil32023
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of DNA
Visualization Methods
EGFP
Processing History
z stack projection
Sample Preparation
Methods
whole mounted tissue
Relation To Intact Cell
living tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 508px ——
Y 512px ——