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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:2881*  Cite 

High resolution image of typical cytoplasm of Coleps hirtus. The cytosol contains rough ER, mitochondria with tubular cristae, peroxisomes, early endosomes, and bacterial endosymbionts. Small and large extrusomes are also present. The bacteria are contained in vacuoles bearing ribosomes on their cytosolic side. Elements of the outer cortex: alveoli, plasma membrane, inner alveolar membrane, outer alveolar membrane are evident as well as the Golgi complex. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/23/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with a Nikon Coolscan 9000ED. This high resolution image is suitable for quantitative analysis Additional information available at (

Biological Sources
NCBI Organism Classification
Coleps hirtus
Cell Type
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component
rough endoplasmic reticulum membrane
smooth endoplasmic reticulum
plasma membrane
symbiont-containing vacuole
bacterial symbionts
Biological Context
Biological Process
regulation of cellular metabolic process
mitochondrial activity
detection of symbiotic bacterium
protein synthesis
detection of symbiont
Molecular Function
cytoplasmic activity
Richard Allen
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 5582px 1nm
Y 3764px 1nm