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CIL:28775*  Cite 

Spinning disk confocal time-lapse imaging of mitosis in asynchronous HeLa cells transfected with control, nontargeting siRNA oligonucleotides, or siRNA oligonucleotides targeting HsKNL2. Top, nontargeting siRNA oligonucleotides; bottom, siRNA oligonucleotides targeting HsKNL2. DIC is shown on the left, and YFP-CENP-A is shown on the right. The control cell on the left begins in metaphase and, over the course of the video, completes mitosis successfully. Paired YFP-CENP-A loci oscillate during metaphase and then segregate to opposite poles during anaphase. In the HsKNL2 knockdown cells, YFP-CENP-A is not seen to localize at any point during the video. Two cells begin in metaphase and leave mitosis during the video. The DIC channel shows that chromosomes do not oscillate as seen in controls and, during anaphase, segregate with aberrant poleward movement in the cell on the left and nominal poleward movement in the cell on the right. The video is ∼75 min long, and the frame is ∼40 µm top to bottom. Images were acquired at 60-s intervals).

Technical Details

Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2. Strain OD31 expressing GFP–KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
Cellular Component
nuclear chromosome
Biological Context
Biological Process
Paul S. Maddox
Francie Hyndman
Joost Monen
Karen Oegema
Arshad Desai
J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 377px ——
Y 304px ——
Time 10 seconds 83