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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:2866*  Cite 

High resolution micrograph through an oblique section of the cortex of Coleps showing many ribosomes, ordered into whorls and spirals, collectively called polysomes, on the surface of the rough endoplasmic reticulum. Mitochondria with tubular cristae and small discoidal vesicles thought to be endosomes are seen. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/23/69 by R. Allen with Philips 300 operating at 60kV.. Neg. 18,500X. The raw film was scanned with a Nikon Coolscan 9000ED. This high resolution image is suitable for quantitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Coleps hirtus
Cell Type
eukaryotic cell
Eukaryotic Protist
CIliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component
cell cortex
rough endoplasmic reticulum
plasma membrane
Biological Context
Biological Process
polysome complexes
vesicle targeting, rough ER to cis-Golgi
Molecular Function
protein synthesis
Richard Allen
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 5582px 0.8nm
Y 3782px 0.8nm