Alternate header for print version

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:27229*  Cite 

Collagen fibrils and mineralization in the external layer of Fundulus heteroclitus scale. The external layer is the second of three layers closest to the dermis of an elasmoid. It is composed of thin collagen fibrils about 30nm in diameter, which are organized in a loose meshwork. This layer is the first layer to be formed and mineralized. This mineralization is visible in the extracellular material surrounding the collagen fibrils where mineral deposits in the form of needle-like crystals identified to most likely be hydroxyapatite can be found.

Technical Details

Fundulus heteroclitus scales were chemically fixed with 2.5% glutaraldehyde, 2% formaldehyde in 0.1M cacodylate buffer (pH 7.3), then post-fixed in 4% osmium tetroxide and stained en bloc in 1% uranyl acetate. The scales were then dehydrated in a graded series of ethanol and infiltrated with Spurr’s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph was imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80 kV.

Biological Sources
NCBI Organism Classification
Fundulus heteroclitus
Cell Type
Cellular Component
fibrillar collagen
mineralization in external layer
Biological Context
Biological Process
extracellular matrix organization
Wai Yan Lam
Marian Rice
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
lead salt
Processing History
unprocessed raw data
Spatial Axis Image Size Pixel Size
X 1689px 1.87nm
Y 1798px 1.87nm