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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:27228*  Cite 

Rough endoplasmic reticulum and Golgi in the epidermal cell of Fundulus heteroclitus scale. Endoplasmic reticulum studded with protein-manufacturing ribosomes form a continuous network along the periphery of this section of the cell. Along the surface of some of the ER folds are energy-generating mitochondria. Deeper into the cell are stacks of cisternae forming the Golgi. Vesicles packaged by the Golgi are visible at the peripheries of these stacks and are found scattered throughout the cytoplasm.

Technical Details

Fundulus heteroclitus scales were chemically fixed with 2.5% glutaraldehyde, 2% formaldehyde in 0.1M cacodylate buffer (pH 7.3), then post-fixed in 4% osmium tetroxide and stained en bloc in 1% uranyl acetate. The scales were then dehydrated in a graded series of ethanol and infiltrated with Spurr’s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph was imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80 kV.

Biological Sources
NCBI Organism Classification
Fundulus heteroclitus
Cell Type
epidermal cell
Cellular Component
rough endoplasmic reticulum
Golgi apparatus
Biological Context
Biological Process
cytoskeleton organization
Wai Yan Lam
Marian Rice
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
lead salt
Processing History
unprocessed raw data
Spatial Axis Image Size Pixel Size
X 1715px 1.92nm
Y 1815px 1.92nm