Autofluorescence from unidentified pollen (mixed pollen grain w.m.; Carolina, commercial sample), presented as a maximum intensity projection obtained from a color-coded Z-stack with 200 image planes. The colors represent the z-position within the maximum intensity projection.
Pollen was imaged using an iMIC Andromeda Spinning Disk Confocal (TILL Photonics GmbH Objective: Olympus UPlanSApo 60X/NA1.35 CCD Camera: Imago QE (PCO). Excitation, 561nm Laser, with the following filters: Laser CleanUp, FF01-446/523/600/677 (Semrock); Dichroic, zt405/490/561/640rpc (Chroma, custom-made design); and emission filter, FF01-390/482/563/640 (Semrock). The colors represent the z-position within the maximum intensity projection. All image processing was done using FIJI. After recording the z-stack (see accompanying raw data in this image group), every focal plane was colorized using the full rainbow, resulting in an RGB z-stack. The maximum intensity projection then produces a colorized max intensity projection. To access FIJI go to http://pacific.mpi-cbg.de/wiki/index.php/Fiji