Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:26592^* Cite

Description

DRB-treated HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein U1-70K and imaged witb a PicoQuant MicroTime 200 confocal microscope using an Olympus 60x 1.2NA water immersion objective lens. DRB inhibition of transcription results in enlargement and rounding up of splicing factor compartments in which the U1-70K is enriched. From Fig 2C in Huranova et al. 2010 J Cell Biol 191:75-86.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: cervical epithelium
Cell Line: HeLa
Cellular Component: pre-mRNA; snRNP; spliceosomal complex

Biological Context

Biological Process: RNA splicing; RNA metabolic process

Attribution

Names: M. Huranova; I. Ivani; A. Benda; I. Poser; Y. Brody; M. Hof; Y. Shav-Tal; K.M. Neugebauer; D. Stanek
Published: M Huranova et al. 2010 J Cell Biol 191:75-86

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL26592
Archival Resource Key (ARK): ark:/b7295/w9cil26592

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: line averaging
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	——
Y	512px	——