Image shows a z-series of a mouse ES cell carrying an alpha-globin BAC transgene array (clone 1c) hybridized with probes against the lac operator repeat (green) and the entire alpha-globin BAC sequence (red). Lac operator sequences formed foci which assumed a more interior location within the engineered chromosome subterritory formed by the BAC transgene array. DAPI counterstaining is shown in blue. Pixel sizes (xy) were 0.074 microns and z-focus steps were 0.2 microns.
Formaldehyde fixed cells were imaged using an IMT-2 Olympus fluorescence microscope with 60x 1.4 NA objective lens. Z-series were recorded, and the slices processed by iterative constrained deconvolution. See also: P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776.
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