When the antigen was visualized with immunogold using DS-1 on thin-frozen sections the antigen could be seen to be present on the periphery of the decorated tubules. The collecting canal and smooth spongiome were not labeled with this mAb. Please see the paper for details. TEM taken on 3/22/86 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5 µm. Adapted with permission from J. Cell Sci. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are outlined in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:1312) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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