The Cell: An Image Library - Image CIL:25846

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CIL:25846^*

Description

Time-lapse of a CHO.K1 cell cotransfected with very low levels of GFP-MIIA (myosin IIA, green) and paxillin (magenta). Yellow arrowheads mark paxillin-containing adhesions that disassemble concomitantly to their MIIA-decorated bundle (marked with arrows) as the movie progresses. Total internal reflection fluorescence (TIRF) images were acquired on an inverted microscope (IX70; Olympus) with a 1.45 NA (oil) Plan-Apochromat 60× TIRFM objective lens. Images were acquired with a charge-coupled device (CCD) camera (Retiga Exi; Qimaging). Video corresponds to Fig 5C and Video 5 from J Cell Biol 2011 193:381-396.

Biological Sources

NCBI Organism Classification: Cricetulus griseus; (Chinese hamsters)
Cell Type: epithelial cell
Cell Line: CHO-K1
Cellular Component: focal adhesion; myosin II complex

Biological Context

Biological Process: focal adhesion disassembly

Attribution

Names: Miguel Vicente-Manzanares; Karen Newell-Litwa; Alexia I. Bachir; Leanna A. Whitmore; Alan Rick Horwitz
Published: J Cell Biol 2011 193:381-396
Pubmed: 21482721

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: TIRF
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP; mCherryFP
Processing History: unproc

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	248px	——
Y	258px	——
Time	5 sec

*CIL – Cell Image Library accession number. Please use this to reference an image.