Description

Polarized cell rearrangement in the Platynereis neuroectoderm visualized by time-lapse imaging. This time-lapse movie shows convergent extension by mediolateral cell intercalation of the ventral neuroectoderm of Platynereis between 53 and 58 hours post fertilization at 25C. The movie covers a period of 300 min in 61 frames and is Movie 1 in supporting information for Proc Natl Acad Sci (2007) 104: 2727-2732.

Technical Details

To label cellular outlines, embryos were incubated for 15 min in 5 μM BODIPY564/570 coupled to propionic acid (Invitrogen, Carlsbad, CA) in natural seawater, then rinsed twice in natural seawater and mounted in a 1:1 mixture of natural seawater and 7.5% MgCl2 to prevent muscular contractions. Time-lapse recordings were performed with a PerkinElmer (Wellesley, MA) Ultraview RS System (spinning disk confocal). Embryos were kept during recordings (12 frames per hour) at a constant temperature of 25C or 26C in natural seawater between slide and coverslip separated by two layers of adhesive tape and silicone paste. The chamber was sealed with mineral oil (Sigma, St. Louis, MO).