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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25690*  Cite 
Description

A view of the entry to the oral apparatus of the green Trichodina that lives on the surface of a freshwater snail collected in central Illinois. In this view the haplokinety and the polykinety of the oral apparatus is cut transversely and an endosymbiotic zoochlorellae are evident. In the case of the haplokinety, there is a gradual view of cross-sections from the axoneme of the cilium through the transition zone to the upper part of the basal body. The haplokinety is distinguished by a single row of ciliated basal bodies and the adjacent outer polykinety is composed of three rows of ciliated basal bodies. The cortical underpinning of the oral cortex is evident. TEM taken by G. Antipa in 1968 with a Hitachi HU11A operating at 75kV. The negative magnification is 7,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.

Biological Sources
NCBI Organism Classification
Trichodina
Chlorella
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
symbiont-containing vacuole
zoochlorellae
oral apparatus
cortical cytoskeleton
cytoplasm
cilium axoneme
microtubule basal body
Attribution
Names
Gregory Antipa (San Francisco State University)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25690
Archival Resource Key (ARK)
ark:/b7295/w9cil25690
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 6042px 1.7nm
Y 5136px 1.7nm