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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25615*  Cite 
Description

Transient release of a cluster of tissue-bound blood cells into circulation in a Drosophila third-instar larva. Real-time movie of blood cells visualized from dorsal side of a Nrg-GFP;PxnYFP larva. A sessile cluster of blood cells breaks apart and some of the cluster enters circulation and flows posteriorly and laterally. However, 4 s later, the released cells reattach to a nearby tissue (tracheal dorsal trunk). Red arrow in frame 1 shows the trajectory of the released cells. Time between frames, 0.325 s. Total duration, 8 s. Fig. 1H was generated from individual frames of this movie. Movie is Movie S7 of Proc Natl Acad Sci (2008). 105: 10017-10022. Movies from this publication include CIL #s 25607, 25608, 25609, 25610, 25611, 25612, 25613, 25614, 25615, 25616, 25617.

Technical Details

Third-instar [w, Nrg-GFP;Pxn-Gal4, UAS-2xeYFP] larvae were mounted dorsal side up on a glass slide with the anterior and posterior ends of the larvae taped down to varying degrees to prevent locomotion and constrain body peristalsis. Larvae were imaged on a Leica MZ16FA fluorescent stereomicroscope using a Planapo 1.0× objective, and images were captured on a monochrome Leica DFC350FX digital camera. For real-time recording, frames were captured every ≈400 ms over a 4- to 40-s period. Images were obtained by using the Scope-Pro Advanced Acquisition plug-in, and image analysis was carried out with Image-Pro AMS ver. 5.1 Software (Media Cybernetics). The object-tracking tool was used to manually track specific blood cells in a time series. Velocities of tracked cells were calculated by using coordinates provided by the software.

Biological Context
Biological Process
innate immune response
wound healing
Attribution
Names
Daniel T. Babcock
Amanda R. Brock
Greg S. Fish
Yan Wang
Laurent Perrin
Mark A. Krasnow
Michael J. Galko
Published
Proc Natl Acad Sci (2008). 105: 10017-10022.
Pubmed
18632567
Link
http://intl.pnas.org/content/105/29/10017.ful...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25615
Archival Resource Key (ARK)
ark:/b7295/w9cil25615
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EYFP
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——
Time 0.325 seconds 25