Description

Blood-cell flow in dorsal region of a Drosophia third-instar larval body. Live visualization of blood cell flow in a Nrg-GFP;PxnYFP larva viewed dorsally. Most blood cells flow from anterior (Upper) to posterior as individual cells or small clusters. Many disappear from view as they flow under the fat body in the middle of the field. Tissue-bound blood cells attached to the body are sessile. Time between frames, 0.34 s. Total duration, 33 s. Fig. 1B was generated from still frames of this movie. Movie is Movie S2 of Proc Natl Acad Sci (2008). 105: 10017-10022. Movies from this publication include CIL #s 25607, 25608, 25609, 25610, 25611, 25612, 25613, 25614, 25615, 25616, 25617.

Technical Details

Third-instar [w, Nrg-GFP;Pxn-Gal4, UAS-2xeYFP] larvae were mounted dorsal side up on a glass slide with the anterior and posterior ends of the larvae taped down to varying degrees to prevent locomotion and constrain body peristalsis. Larvae were imaged on a Leica MZ16FA fluorescent stereomicroscope using a Planapo 1.0× objective, and images were captured on a monochrome Leica DFC350FX digital camera. For real-time recording of larval circulation, frames were captured every ?400 ms over a 4- to 40-s period. Images were obtained by using the Scope-Pro Advanced Acquisition plug-in, and image analysis was carried out with Image-Pro AMS ver. 5.1 Software (Media Cybernetics).