Loose tethering of blood cells adherent to the unwounded body wall of a Drosophila third-instar larva. Real-time movie of blood cells seen from the dorsal side of a Nrg-GFP;PxnYFP larva. A cluster of three cells (circled in frame 1) is tethered to the body wall at a single point around which the cluster pivots as blood flows past. Time between frames, 0.325 s. Total duration, 4 s. Fig. 1G was generated from individual frames of this movie. Movie is Movie S1 of Proc Natl Acad Sci (2008). 105: 10017-10022. Movies from this publication include CIL #s 25607, 25608, 25609, 25610, 25611, 25612, 25613, 25614, 25615, 25616, 25617.
Third-instar [w, Nrg-GFP;Pxn-Gal4, UAS-2xeYFP] larvae were mounted dorsal side up on a glass slide with the anterior and posterior ends of the larvae taped down to varying degrees to prevent locomotion and constrain body peristalsis. Larvae were imaged on a Leica MZ16FA fluorescent stereomicroscope using a Planapo 1.0× objective, and images were captured on a monochrome Leica DFC350FX digital camera. For real-time recording of larval circulation, frames were captured every ≈400 ms over a 4- to 40-s period. Images were obtained by using the Scope-Pro Advanced Acquisition plug-in, and image analysis was carried out with Image-Pro AMS ver. 5.1 Software (Media Cybernetics).
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