Alternate header for print version

This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25538*  Cite 

A 3D reconstruction of the cardiac outflow tract (OFT) region (horizontal and vertical rotation) from stage-14 wild-type embryo stained for Tinman (Tin; red) and Msp300 (green). Tin staining reveals the cardiac cells and, located ventrally to cardiac outflow muscles (COM), cvr cells (red cells without Msp300 staining). The two anterior cardioblasts coexpressing Tin and Msp300 (ACB cells) are seen associated with COM (refer to Fig. 1). Notice the position of ACBs linking the two COMs (frontal view) and located dorsally with respect to COM fibers (lateral view). Movie is SI Movie 2 in Proc Natl Acad Sci USA (2008) 105: 2475-2480. Movies from this publication include CIL# 25536, 25537, 25538, 25539, 25540, 25541.

Technical Details

Stage 14 embryos were stained with primary antibodies: rabbit anti-Tim, guinea pig anti-Msp300. Species-specific secondary antibodies were conjugated to Alexa Fluor 488, Cy3 or Cy5. The double stained embryos were visualized using a Zeiss LSM 510 Meta confocal microscope. Images representing 0.5 mm optical sections were compiled and processed by Volocity software (Improvision).

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
cardioblast (sensu Arthropoda)
pericardial cell
cardiac outflow muscle
heart-anchoring cells
cephalic vascular rudiment cells
Cellular Component
actin cytoskeleton
integrin complex
Monika Zmojdzian
Jean Philippe Da Ponte
Krzysztof Jagla
Proc Natl Acad Sci USA (2008) 105: 2475-2480.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
3D reconstruction
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
3D reconstruction
Data Qualifiers
processed data
Sample Preparation
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 784px ——
Y 576px ——
Z —— 500µm