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Description
Electron micrograph of Maize mosaic virus (MMV, Rhabdoviridae) in the accessory salivary gland of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. MMV buds through nuclear membranes and accumulates in perinuclear space in cells of the accessory salivary gland. The accompanying image in this image group includes labels and scale bar. Methods: Dissected insect organs were processed for TEM by fixation in glutaraldehyde and osmium tetroxide and embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM. Associated references: Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85. Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41.
Biological Sources
- NCBI Organism Classification
- Maize mosaic virus
- Peregrinus maidis
- Cell Type
- secretory cell
- Cellular Component
- nucleus
- nuclear membrane
- host cell perinuclear region of cytoplasm
Biological Context
- Biological Process
- viral transmission by vector
- interspecies interaction between organisms
Attribution
- Names
- El-Desouky Ammar
- Saskia A. Hogenhout
- L. R. Nault
Grouping
This image is representative of a group of similar images.Imaging
- Image Type
- transmission electron microscopy (TEM)
- Imaging Mode
- detection of electrons
- illumination by electrons
- Parameters Imaged
- electron density
- Source of Contrast
- stain with broad specificity
- Visualization Methods
- uranyl salt
- lead salt
- Processing History
- unprocessed raw data
- Data Qualifiers
- raw, unprocessed data
- suitable for spatial measurements
Sample Preparation
- Methods
- osmium tetroxide fixed tissue
- glutaraldehyde fixed tissue
- tissue in plastic embedment
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 797px | 0.01μm |
| Y | 1006px | 0.01μm |