Licensing

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Description

Electron micrograph of Maize mosaic virus (MMV, Rhabdoviridae) in the accessory salivary gland of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. MMV buds through nuclear membranes and accumulates in perinuclear space in cells of the accessory salivary gland. The accompanying image in this image group includes labels and scale bar. Methods: Dissected insect organs were processed for TEM by fixation in glutaraldehyde and osmium tetroxide and embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM. Associated references: Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85. Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41.

Biological Sources

NCBI Organism Classification
Maize mosaic virus
Peregrinus maidis
Cell Type
secretory cell
Cellular Component
nucleus
nuclear membrane
host cell perinuclear region of cytoplasm

Attribution

Names
El-Desouky Ammar
Saskia A. Hogenhout
L. R. Nault

Grouping

This image is representative of a group of similar images.

Imaging

Image Type
transmission electron microscopy (TEM)
Imaging Mode
detection of electrons
illumination by electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
uranyl salt
lead salt
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Dimensions

Spatial Axis Image Size Pixel Size
X 797px 0.01μm
Y 1006px 0.01μm
*CIL – Cell Image Library accession number. Please use this to reference an image.