QuickTime movie of a complete optical scan through a rabbit intestinal villus tip infected with infected with 10× the amount (4 × 108 CFU/ml) of ΔinlA L. monocytogenes (green) than shown for Wt-GFP L. monocytogenes, and counterstained with phalloidin (red) to visualize the actin cytoskeleton, and with toto-3 (blue) to visualize nuclei. Paraformaldehyde fixed intact tissue blocks were processed without prior embedding and sectioning. The samples were imaged by confocal microscopy where optical sections were taken at 0.5 micron resolution through both the cell monolayers and the intestinal villi. Z-stacks were reconstructed into three dimensions using Volocity software. Corresponds to Video 4 from PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27.
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