Reorganization of cells around a site of cell extrusion monitored live by time-lapse differential interference contrast microscopy. The process of extrusion began at hour 1 of observation and continued for 40 min. Monolayer was imaged on a Zeiss Axiovert 200M microscope using a 40× dry differential interference contrast microscopy (DIC) objective. Images were collected by a charge-coupled device camera (CoolSNAP-HQ, Photometrics, Tucson, AZ) and stored digitally every 10 min. Image corresponds to Fig 4 and Video 1 in PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27.
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