The Cell: An Image Library - Image CIL:25304

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CIL:25304^*

Description

Confocal fluorescence time-lapse imaging of a mitotic Drosophila S2 cell expressing GFP-tubulin and treated with 100 nM taxol for 1 h before imaging to induce assembly of monopolar spindles. Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Supplemental Video 5 in Maresca and Salmon, 2009 J Cell Biol 184:373-281.

Biological Sources

NCBI Organism Classification: Drosophila melanogaster; (Common fruit fly)
Cell Line: S2
Cellular Component: spindle

Biological Context

Biological Process: mitosis

Attribution

Names: Thomas Maresca; Edward Salmon
Published: Maresca and Salmon, 2009 J Cell Biol 184:373-281
Pubmed: 19193623

Imaging

Image Type: charge coupled device (CCD)
Imaging Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: Green fluorescent proteins from Aequorea
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	248px	0.065μm
Y	248px	0.065μm
Time	120 sec	50

*CIL – Cell Image Library accession number. Please use this to reference an image.