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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25304*  Cite 
Description

Confocal fluorescence time-lapse imaging of a mitotic Drosophila S2 cell expressing GFP-tubulin and treated with 100 nM taxol for 1 h before imaging to induce assembly of monopolar spindles. Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Supplemental Video 5 in Maresca and Salmon, 2009 J Cell Biol 184:373-281.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Line
S2
Cellular Component
spindle
Biological Context
Biological Process
mitosis
Attribution
Names
Thomas Maresca
Edward Salmon
Published
Maresca and Salmon, 2009 J Cell Biol 184:373-281
Pubmed
19193623
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25304
Archival Resource Key (ARK)
ark:/b7295/w9cil25304
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 248px 0.065µm
Y 248px 0.065µm
Time 120 seconds 50