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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24967*  Cite 

Fat bodies from 3rd instar larvae of Drosophila melanogaster were dissected, fixed with formalin and stained with uranyl acetate, embedded in epoxy resin, and 0.5 micrometer sections prepared (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV. The image shows a band-interband-band region of polytene chromosome taken with a tilt angle of +5 degrees. The accompanying grouped image, taken at -5 degrees forms a stereo pair, providing a 3D view of the chromosome.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
fat body cell
Cellular Component
polytene chromosome
Hans Ris
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
elastic scattering of electrons
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Sample Preparation
formaldehyde fixed tissue
tissue in epoxy resin embedment
Relation To Intact Cell
microtome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 6600px 1nm
X 4900px 1nm