Fat bodies from 3rd instar larvae of Drosophila melanogaster were dissected, fixed with formalin and stained with uranyl acetate, embedded in epoxy resin, and 0.5 micrometer sections prepared (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV. The image shows a band-interband-band region of polytene chromosome taken with a tilt angle of +5 degrees. The accompanying grouped image, taken at -5 degrees forms a stereo pair, providing a 3D view of the chromosome.
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