Localization of XAC XAC (Xenopus ADF/cofilin) to posterior regions of depolymerization-resistant actin brush. Fluorescence microscopy of lamellipodia of Xenopus keratocytes after latrunculin a (a binder of monomeric actin) treatment (0.25 μM for 10 min) and subsequent staining with XAC antibody and TRITC-phalloidin. Image corresponds to Figure 9b in J Cell Biol. 1999 May 31;145(5):1009-26.
Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).
|Spatial Axis||Image Size||Pixel Size|