Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24809^* Cite

Description

Localization of Xenopus ADF/cofilin (XAC) to posterior regions of depolymerization-resistant actin brush. Electron (micrograph of lamellipodia of Xenopus keratocytes after unprotected extraction and subsequent staining with XAC antibody. Image corresponds to Figure 9a from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: keratocyte
Cellular Component: lamellipodium; actin cytoskeleton; Xenopus ADF/cofilin

Biological Context

Biological Process: cellular localization

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL24809
Archival Resource Key (ARK): ark:/b7295/w9cil24809

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating; platinum replica; primary antibody plus labeled secondary antibody; immuno gold
Processing History: unprocessed raw data

Sample Preparation

Methods: permeabilized tissue; glutaraldehyde fixed tissue
Relation To Intact Cell: dispersed cell in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2070px	——
Y	2618px	——