Differential response of lamellipodial actin network to latrunculin a (LA). Electron micrograph of a Xenopus fibroblast lamellipodium treated with LA (0.25 μM for 10 min) reveals actin depletion from the lamellipodial rear. A similar image of an LA treated keratocyte is available as CIL 24804. Image corresponds to Figure 7e from J Cell Biol. 1999 May 31;145(5):1009-26.
Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).
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