The Cell: An Image Library - Image CIL:24795

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CIL:24795^*

Description

Localization of Arp2/3 complex in the lamellioidia of a Xenopus keratocytes. Staining with p21 antibody and 10 nm gold secondary antibody. CIL 24794 shows a fluorescence microsopy image of Arp2/3 localization in a Xenopus keratocyte. CIL 24796 and 24797 show corresponding images in fibroblasts. Image corresponds to Figure 3d from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis; (African clawed frog)
Cell Type: keratocyte
Cellular Component: lamellipodium; actin cytoskeleton

Biological Context

Biological Process: cellular macromolecule localization

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating; primary antibody plus labeled secondary antibody; immuno-gold
Processing History: unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1022px	4.5nm
Y	1410px	4.5nm

*CIL – Cell Image Library accession number. Please use this to reference an image.